大家都知道，CRISPR/Cas9基因编辑技术在高效获得基因敲除突变（knockout）方面是非常有效的，但是想要通过同源重组修复途径 ...

实现更特异、更高效的基因敲入（knockin）！ CRISPR/Cas9和其它基因编辑工具已经被成功应用于获得基因敲除突变（knockout），但众多事实证明在基因敲入上的应用有很大的难度。基因敲入所面临的主要难题是修复模板的制备和如何与Cas9-sgRNA核糖核蛋白复合物共导入 ...

该研究对用于 CRISPR 基因编辑治疗的 Cas 核酸酶 SaCas9 和 AsCas12a 进行了理性工程化改造，设计出了具有最小化免疫原性的 SaCas9 和 AsCas12a 突变体。

and we succeeded in both large-DNA fragment knockin and whole exon exchange with electroporation of CRISPR/Cas9 ribonucleoprotein. By this method, we can exchange large DNA fragments conveniently in ...

CRISPR-Cas9 genome editing exploits the CRISPR-Cas system to modify a genome in a targeted manner. Guided by RNA, the Cas9 endonuclease breaks DNA at a target sequence. Imprecise repair of the ...

This repository includes a recurrent convolutional neural network named CRISPR-Net for predicting the off-targets activities with insertions, deletions and mismatches in CRISPR/Cas9 gene editing.

CRISPR Therapeutics' stock decline is likely influenced by Editas Medicine's struggles, but CRISPR remains a distinct and stronger company with a promising long-term outlook. CRISPR's strategic ...

Using “Easi-CRISPR” (Quadros RM, Genome Biology, 2017, PMID: 28511701), we can now rapidly and easily generate Cre knockin mice, as well as many other types of mice, via one-step zygote injection of ...

163.com or Ji Li-Juan, Department of Rehabilitation, The Second People's Hospital of Huai'an, Huai'an, China. 189{at}whu.edu.cn First introduced into mammalian organisms in 2013, the RNA-guided genome ...

CRISPR is a technology that can be used to edit genes and, as such, will likely change the world. The essence of CRISPR is simple: it’s a way of finding a specific bit of DNA inside a cell.