In somatic cells, these phases are separated by gaps: G1 (unreplicated chromosomes) and G2 (replicated ... in the mammalian cell cycle is another irreversible transition (G0–G1) that can be ...

This sequential expression followed the phases of the traditional cell cycle: G0/G1 (the cell grows larger), S (the cell replicates its DNA), and G2/M (the cell prepares for division and then splits).

The stain can be applied to live, detergent-permeabilized or fixed cells for quantification of their distribution among the three main phases of the cell cycle (G 0 /G 1, S and G 2 /M).