Plasmid vectors pET11a-link-NGFP and pMRBAD-link-CGFP and positive control vectors pET11a-Z-NGFP and pMRBAD-Z-CGFP are available upon request (Fig. 1) Figure 1: GFP-fragment reassembly vectors and ...
The gene coding for GFP comes from the bioluminescent jellyfish, Aequorea victoria, and resides on a DNA plasmid called pGLO. The pGLO plasmid also contains a gene encoding for beta-lactamase.