At 48-72 hours post-transfection, the supernatant (containing the viral particles) is harvested and clarified. At this point lentiviral vectors can be used to transduce the mammalian cell line of ...

Recombinant adeno-associated virus (AAV) and lentiviral vectors (LV) are vehicles for direct delivery of therapeutic ... In this talk, we present optimization strategies for the transfection step of ...

541 671 CRISPR cell line (Transient transfection) extra order/on-target KO line) 221 274 CRISPR cell line (Lentiviral delivery) (1 ctrl line + 1 on-target KO line) 585 726 CRISPR cell line (Lentiviral ...