Recombinant adeno-associated virus (AAV) and lentiviral vectors (LV ... In this talk, we present optimization strategies for the transfection step of vector production to generate high titers of both ...

At 48-72 hours post-transfection, the supernatant (containing the viral particles) is harvested and clarified. At this point lentiviral vectors can be used to transduce the mammalian cell line of ...

They are ideal for labeling cell types that are difficult to label using BacMam, lentivirus or other transfection-based labeling approaches (e.g. immune cells and primary cells). These dyes can be ...

541 671 CRISPR cell line (Transient transfection) extra order/on-target KO line) 221 274 CRISPR cell line (Lentiviral delivery) (1 ctrl line + 1 on-target KO line) 585 726 CRISPR cell line (Lentiviral ...