Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. Genomic DNA is assembled into chromatin by ...
Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. Impaired insulin receptor signaling is ...
(D) Targets are highly concentrated around the beads, ensuring that each micrograph contains more than 100 usable particles for 3D structure determination. The fraction with the interphase-specific ...
The main strength of this work is a clever and novel methodological design, in particular the engineered protein spacers to separate captured nucleosomes from streptavidin beads for a clear imaging.
Uncoated beads were used as a negative control. The A20 D1.3 cells transfected with GFP-VTI1b or GFP as control were mixed with the anti-IgM-coated and uncoated beads (1:1) and plated on ...
The GFP fluorescence was excited by the 488 nm line of a laser ... 150–250 mg of plant tissue was homogenized in tubes with 1 g of 1.3 mm silica beads using a FastPrep-24 instrument (MP Biomedicals, ...