For assays that use an internal standard, running a standard for each sample would be cumbersome, and would consume large quantities of reagents. It is standard practice to use a one point standard to calculate your values.

Simple. Using our internal standard method and robust reagents, no sophisticated sample pretreatment is required. Sensitive and accurate. Use as little as 10 μL samples. Linear detection range in 96-well plate: 0.066 to 3 mM for colorimetric assays. High-throughput. Can be readily automated to assay thousands of samples per day. Method ...

Samples requiring an internal standard will need two separate reactions: 1) Sample plus Internal Standard, and 2) Sample alone. For the sample plus standard well, add 20 µL of 1 mM AAT Standard

You can try using an internal standard with biological matrix you are testing. To do this, you will employ three different wells, one with Sample, one with Sample plus Internal Standard, and one that will be the Sample Blank.

Internal standard is required for colorimetric assay. Each sample requires two separate reactions: 1) Sample plus internal standard and 2) Sample alone. In addition, each assay plate requires a water blank well. Add 20 µL of each sample to two separate wells. Also, add 20 µL dH 2O to a separate well. For the internal standard, prepare 400 µL 1

Note: The volume of the internal standard is 4× lower than the sample volume; thus, the internal standard concentration should be divided by 4. What samples have you tested? This kit has been tested on serum, culture media, mammalian cell lysates, …

500 µM is the effective concentration of the Internal Standard, and n is the dilution factor. Note: if the Sample oxalate concentration is higher than 1000 µM, dilute