Emerging nanomaterials for targeting peroxisomes - ScienceDirect
2022年8月1日 · Only a handful of studies have reported nanomaterials such as quantum dots, zeolites, liposomes, dendrimers, and nanoparticles to detect and fabricate peroxisomes. This …
Dynamic assembly of DNA-ceria nanocomplex in living cells
2022年12月14日 · Herein, we design a DNA-ceria nanocomplex (DCNC) to achieve intracellular dynamic assembly, and thus realize the in situ construction of artificial peroxisomes, which …
Correlative Light- and Electron Microscopy in Peroxisome …
Correlative light and electron microscopy (CLEM) combines the advantages of protein localization by fluorescence microscopy with the high resolution of electron microscopy. Here, we describe …
Peroxisome inspired hybrid enzyme nanogels for …
2021年9月2日 · Bioinspired by the H 2 O 2 activation reaction and the O 2 redistribution pathways of peroxisome, herein as a proof of concept, we design and develop an enzyme complex …
Morphology of Peroxisomes in Light- and Electron Microscopy
The development of the alkaline 3,3′-diaminobenzidine (DAB) method for the light- and electron microscopical visualization of the peroxidatic activity of catalase (a marker enzyme of POs)[10, …
Ultrastructural Analysis and Quantification of Peroxisome …
2023年3月24日 · Peroxisomes fulfil complex cellular tasks by establishing close inter-organelle membrane contact sites with other compartments of the cell, leading to the exchange of …
Visualization and Quantitation of Peroxisomes Using Fluorescent ...
2004年4月14日 · Typically, liver peroxisome proliferation has been estimated by direct counting via electron microscopy, as well as by gene expression, enzyme activity, and immunolabeling. …
Ultrastructural Analysis and Quantification of Peroxisome …
Hacker C, Asadi J, Pliotas C et al (2016) Nanoparticle suspensions enclosed in methylcellulose: a new approach for quantifying nanoparticles in transmission electron microscopy. Sci Rep …
Peroxisomes: Methods and Protocols | SpringerLink
Chapters in Peroxisomes: Methods and Protocols illustrate the isolation of peroxisomes, investigation of properties of membrane proteins, protocols to investigate and manipulate …
Cryo-Immuno Electron Microscopy of Peroxisomal Marker Proteins
We will describe here the variant and common steps of both methods in detail and illustrate their potency in the ultrastructural imaging of peroxisomes. Keywords: Cryo-immuno electron …