In biochemistry, a protein trimer is a macromolecular complex formed by three, usually non-covalently bound, macromolecules like proteins or nucleic acids. A protein trimer often occurs from the assembly of a protein's quaternary structure. [1]

2020年7月22日 · We demonstrate that the designed, thermostable, closed S trimer can be used in serological assays. This protein has potential applications as a reagent for serology, virology and as an immunogen.

The folding and oligomerization of coronavirus spike protein were explored using a panel of monoclonal antibodies. Chemical cross-linking and sedimentation experiments showed that the spike of transmissible gastroenteritis virus is a homotrimer of the S membrane glycoprotein.

2021年1月11日 · Here, Juraszek et al. present a stable SARS-CoV-2 S-closed protein variant with increased expression and correct folding, predominantly in closed prefusion conformation.

S protein derivatives are components of vaccine candidates and diagnostic assays, as well as tools for research into the biology and immunology of SARS-CoV-2. Here we have designed mutations in S that allow production of thermostable, disulphide-bonded S protein trimers that are trapped in the closed, prefusion, state.

2022年9月17日 · In the present study, we showed that HR-fused RBD protein that contains both L452R and T478K mutations could self-assemble into trimers, and elicit high levels of neutralizing antibodies to...

Using Trimer-Tag technology (8), we produced both soluble wild-type (WT) and a furin site mutant (MT) (R685A) forms of S-Trimer from CHO cells in serum-free fed-batch processes in bioreactors, with protein yields ranging from 0.5 to 1 g/liter.

We determined cryo-EM structures of the SARS-CoV-2 S ectodomain trimer, providing a blueprint for the design of vaccines and inhibitors of viral entry.

2011年12月5日 · Detailed characterization of AcrB P223G revealed that the protein existed as a well-folded monomer after purification, but formed a trimer in vivo. The function of the mutant could be partly restored through strengthening the stability of …

2020年9月30日 · The vaccine established using S trimer protein in the prefusion state could help protect humans from SARS-CoV-2 attack since the virus is in the prefusion state when they …