2023年8月21日 · Stable cell lines play a crucial role in advancing scientific research, enabling prolonged and consistent gene expression for various applications. This article provides a comprehensive guide for researchers seeking to establish stable cell lines that constitutively express GFP-tagged proteins.

2023年9月9日 · This protocol can be used to generate stable cell lines expressing a gene of interest from an integrated lentiviral vector. Unlike the short-term protein expression observed using transient transfection approaches, generating cell lines using lentiviral vectors enables long-term protein expression studies.

Stable cell lines are cellular models that harbor genetic modifications to express specific proteins or biomolecules of interest. Generating stable cell lines involves a sequential series of events intended to produce a uniform population of cells that homogeneously express the transgene.

The generation of stably-transfected cell lines is essential for a wide range of applications, such as gene function studies (Grimm, 2004), drug dis- covery assays or the production of recombinant proteins (Wurm, 2004).

Explore stable transfection for the introduction of DNA into cells long-term, including selection markers and antibiotics for stable cell line generation.

Lentiviruses are used very widely to generate stable expression mammalian cell lines. They are used for both gene down-regulation (by using shRNA) or for gene up-regulation (by using ORF of gene of interest).

2024年8月23日 · Learn the essentials of stable cell line generation, from vector design to clone selection, and enhance your biomanufacturing success with expert tips and strategies

There are two types of stable cell lines. One is achieved by eukaryotic vectors that harbor elements for episomal maintenance in the nucleus of a transfected cell. Another is achieved via direct integration of the transfected plasmid into the target cells genome.

Scientists use stable cell lines for diverse applications, ranging from studying cell differentiation, gene expression, cellular events, toxicity, and production of recombinant antibodies and proteins. Stable cell lines are also helpful in the evaluation of drugs in specific environments.

Stable cell lines contrast strongly with transiently-transfected cells, which do not have stable genetic and protein expression characteristics. Transient transfection results in temporary changes to cell lines, which may aid in one-time production of proteins or short-term experiments.